Fig. 5

The inhibitory effects of BP on the migration and invasion of human bladder cancer cells- trans-well assay. a Human bladder cancer cells were pretreated with 0.2% DMSO as control or 60 μg/ml BP for 24 h and then seeded onto the trans-well hanging insert coated with (invasion) or without (migration) 50 μg/ml Matrigel for different time points (5637: 16 h, BFTC: 2 h, T24: 4 h and TCCSUP: 6 h). Images were were captured using inverted microscope with a 200 X magnification; Scale bar: 50 μm. Quantitative data are shown by histograms of migrated/invaded cells from different treatment. b Human bladder cancer cells were treated with 60 μg/ml BP for 24 h, and the mRNA expression levels of E-cadherin and N-cadherin were determined by real-time RT-PCR analysis. Data are presented as means ± S.D. from three different experiments. * p < 0.05 versus vehicle; ** p < 0.01 versus vehicle