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Fig. 6 | BMC Complementary and Alternative Medicine

Fig. 6

From: Antiplatelet mechanism of an herbal mixture prepared from the extracts of Phyllostachys pubescens leaves and Prunus mume fruits

Fig. 6

Effects of PM21 on collagen-induced PLCγ2 and PI3 K activation. Rat platelets in the presence of EGTA were incubated with PM21 and then stimulated with collagen. a Lysates from the platelets treated with either PM21 (50, 100, 200 μg/mL) or aspirin (50 μg/mL) were immunoblotted to detect the expression levels of PLCγ2 and PI3 K (p85) proteins. b Platelet lysates were immunoprecipitated by incubating overnight with anti-PI3 K or anti-PLCγ2, and then further incubated with protein A-Sepharose (PAS). Precipitated proteins were separated by SDS-PAGE and immunoblotted to detect phosphotyrosine residues. Equivalent protein loading was verified by reprobing for PLCγ2 and PI3 K (p85). IP and IB in the diagram represent Immunoprecipitated and Immunoblotted, respectively

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