Fig. 3
Effects of PM21 on collagen-induced fibrinogen binding to integrin αIIbβ 3. Rat platelets were pre-incubated for 2 min with various concentrations of PM21 (50, 100, 200 μg/mL), then stimulated with 5 μg/mL collagen in the presence of Ca2+ (1 mM) for 5 min, and immediately incubated thereafter with fibrinogen Alexa Fluor 488 (20 μg/mL) for 5 min. Nonspecific binding of fibrinogen to integrin αIIbβ3 was assessed in the presence of the calcium chelator, EGTA (1 mM). Fibrinogen Alexa Fluor 488 conjugate binding to platelets was quantified by flow cytometry. Data show the mean ± SEM of at least three independent experiments. ** p < 0.01, *** p < 0.001 versus vehicle control