Fig. 7

Effect of COP on cell apoptosis. a U251 and U87 cells were treated with COP at different concentrations (0, 0.625, 1.25 and 2.5 mg/ml) for 24 h, then stained with Annexin V-fluorescein isothiocyanate (FITC)/PI. b Quantitative analysis of dose-dependent alteration of COP on apoptosis. c U251 and U87 cells were treated with COP at different for 24 h, the expression of Caspase 3 were down-regulated and cleaved Caspase 3 were increased. Data are presented as mean ± SD from three independent experiments. *, P < 0.05; **, P < 0.01; ***, P < 0.001 compared with the control group