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Table 2 Induction of different cytochrome P450 isoenzymes in cultured human hepatocytes from five different donors with Helixor® A (HA), Helixor® M (HM) and Helixor® P (HP). Fold induction of activity over respective negative control (incubation with no test item) is given. Test mixtures containing reference inducers were used as positive controls (PC)

From: Effects of mistletoe products on pharmacokinetic drug turnover by inhibition and induction of cytochrome P450 activities

CYP CYP1A2 CYP2B6 CYP2C9 CYP2E1 CYP3A4
Donor D1 D2 D3 D1 D4 D5 D1 D4 D5 D1 D2 D3 D1 D2 D3
PC 19.2 21.8 31.6 3.0 3.7 2.9 1.5 1.3 1.5 1.2 1.0 1.0 1.7 1.9 3.3
HA 10 μg/ml 0.5 0.8 0.4 1.0 0.6 1.2 0.6 0.6 0.4 0.9 0.6 0.8 1.0 0.7 0.9
HA 5 μg/ml 0.6 1.1 0.4 1.0 0.9 1.2 0.6 1.1 1.0 1.0 0.6 1.0 1.4 0.8 0.9
HA 0.5 μg/ml 0.8 1.2 0.4 1.0 1.1 0.8 0.9 1.2 0.4 0.9 0.7 1.0 1.4 0.9 1.1
HM 10 μg/ml 0.6 0.8 0.3 0.6 0.2 0.9 0.5 0.1 0.8 0.9 0.7 0.8 0.7 0.4 0.4
HM 5 μg/ml 0.6 1.0 0.4 1.2 0.8 1.2 0.8 0.7 0.9 1.1 0.9 1.3 1.1 0.5 0.9
HM 0.5 μg/ml 0.8 1.1 0.4 1.3 1.2 1.0 0.9 1.0 1.0 1.1 0.8 1.3 1.4 0.6 1.1
HP 10 μg/ml 0.5 0.3 0.1 0.4 n.d. 0.4 0.4 n.d. 0.1 0.3 0.1 n.d. 0.4 0.1 0.1
HP 5 μg/ml 0.7 0.6 0.4 0.5 0.0 0.9 0.7 0.0 0.2 0.8 0.4 0.6 0.5 0.2 0.3
HP 0.5 μg/ml 0.8 0.9 0.3 1.3 0.7 1.1 1.1 1.3 1.3 0.9 0.9 1.2 1.0 0.6 1.0
  1. D1, D2, D3, D4, D5: donor 1 to donor 5;
  2. Positive controls (PC): 50 μM omeprazole (1A2); 1 mM phenobarbital (2B6, 2C9); 100 mM ethanol (2E1); 10 μM rifampicine (3A4);
  3. n.d. not detectable