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Table 1 HPLC / MS parameters for marker substrate and marker metabolite separation and detection

From: Effects of mistletoe products on pharmacokinetic drug turnover by inhibition and induction of cytochrome P450 activities

CYP
Iso-enzyme
Marker substrate Marker metabolite Gradient/isocratic Injection
volume
[μl]
Flow
rate
[ml/min]
Column
temp.
[°C]
Stop
time
[min]
Detection
2A6 coumarin umbelliferone 0–6 min linear 10 0.4 30 8 Transition (m/z) 163.0 > 107.0
2B6 S-mephenytoin nirvanol 0–4 min linear 10 0.4 30 6 Transition (m/z) 205.0 > 134.0
2C8 paclitaxel 6α-hydroxypaclitaxel 0–6 min linear 10 0.4 30 7.5 Transition (m/z) 870.2 > 524.9
2C9 diclofenac 4′-hydroxydiclofenac 0–6 min linear 10 0.4 30 7 SIR (m/z) 265.9 / 249.9
2C19 S-mephenytoin 4′-hydroxymephenytoin 0–6 min linear 10 0.4 30 6 Transition (m/z) 235.0 > 150.2
2D6 bufuralol hydroxybufuralol 0–6 min linear 10 0.4 30 8 MRM (m/z) 278.2 > 186.1
2E1 chlorzoxazone 6-hydroxychlorzoxazone Isocratic 20 0.5 30 8 Absorption at 298 nm
3A4 testosterone 6β-hydroxytestosterone 0–6 min linear 10 0.4 30 7.5 Transition (m/z) 305.0 > 287.0
  1. m/z mass-to-charge ratio, SIR selected ion recording, MRM multiple reaction monitoring