Skip to main content

Table 5 Real-time RT-PCR analysis of gene expression level after treating MCF-7 cells with NDL for 24, 36 and 48 h

From: Nam Dia long, a Vietnamese folk formula, induces apoptosis in MCF-7 cells through various mechanisms of action

Gene

Realtime RT-PCR

24 h

36 h

48 h

ER stress

 GRP78

1.02 ± 0.16

10.82 ± 3.05**

10.06 ± 3.05**

 ERN1

1.68 ± 0.32

8.86 ± 6.54*

2.10 ± 0.53*

 ATF4

1.75 ± 0.37*

3.67 ± 0.61**

1.11 ± 0.24

 XBP1

2.71 ± 1.84

2.17 ± 0.06**

0.60 ± 0.24

 DDIT3

0.94 ± 0.59

26.45 ± 14.47*

4.56 ± 1.57*

 FAM129A

4.52 ± 1.84*

44.20 ± 11.70**

34.84 ± 9.14**

 GDF15

5.65 ± 0.72**

35.36 ± 9.23*

19.49 ± 7.78**

p53 pathway

 TP53

2.06 ± 0.95

2.46 ± 0.05***

1.30 ± 0.23

 RELA

0.90 ± 0.32

1.65 ± 0.26*

1.14 ± 0.41

 GADD45A

2.84 ± 1.17

12.82 ± 3.45**

6.15 ± 3.43*

 CDKN1A

0.76 ± 0.46

3.26 ± 0.17***

2.91 ± 0.69*

Cell cycle

 CDK1

0.88 ± 0.12

0.61 ± 0.07*

0.41 ± 0.08*

 CDK2

0.75 ± 0.29

0.64 ± 0.08*

0.37 ± 0.18

 CCNB

0.95 ± 0.33

0.71 ± 0.38

0.19 ± 0.12*

 CCNE

0.70 ± 0.32

1.01 ± 0.26

0.48 ± 0.13*

 CCNA2

0.66 ± 0.33

0.81 ± 0.17

0.50 ± 0.24*

Apoptosis

 BCL2

0.37 ± 0.12*

0.48 ± 0.21

0.25 ± 0.11*

 BAX

0.70 ± 0.23

1.02 ± 0.17

3.28 ± 1.03*

 CAS9

1.30 ± 0.17

1.99 ± 0.87

2.23 ± 0.44*

 CAS8

0.96 ± 0.21

2.52 ± 0.62*

2.30 ± 0.14*

 NOXA

1.29 ± 0.59

4.43 ± 1.75*

0.57 ± 0.40

 PUMA

3.31 ± 3.10

6.76 ± 1.75**

11.95 ± 5.74*

 TRAIL

2.44 ± 1.87

2.44 ± 1.82

1.78 ± 0.03***

Oxidative stress response

 NFE2L2

0.91 ± 0.51

1.53 ± 0.13*

1.05 ± 0.61

 HMOX1

5.69 ± 0.90**

9.37 ± 3.96*

17.40 ± 7.54*

 CAT

1.41 ± 0.33

1.56 ± 0.31

0.64 ± 0.31

 SOD1

1.59 ± 0.43

2.44 ± 0.95

0.50 ± 0.23

 SOD2

1.10 ± 0.37

1.30 ± 0.21

0.85 ± 0.05*

 GPX-1

1.87 ± 0.31*

3.83 ± 2.50

0.80 ± 0.57

Estrogen receptor

 ESR1

0.81 ± 0.06*

0.73 ± 0.11

0.70 ± 0.29

 ESR2

2.08 ± 1.20

2.70 ± 0.61*

2.03 ± 0.58

  1. Each value represents mean ± SD (n = 3). Fold change indicated relative quantitation compared to GADPH. Fold change >1 indicates upregulation while fold change < 1 indicates down regulation. The statistical differences between the treatment and control were analyzed by two-tailed paired Student’s t-tests (*p < 0.05; **p < 0.01; ***p < 0.001)