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Fig. 5 | BMC Complementary and Alternative Medicine

Fig. 5

From: Anti-inflammatory evaluation of the methanolic extract of Taraxacum officinale in LPS-stimulated human umbilical vein endothelial cells

Fig. 5

Effect of the Taraxacum officinale (TO) methanol extract on the LPS-induced nuclear translocation of NF-κB p65 in HUVECs. HUVECs were pretreated with TO extract (100 μg/ml) for 1 h and then stimulated with LPS (1 μg/ml) for 1 h. a Representative images of immunofluorescence staining showing NF-κB p65 (red) and cell nuclei stained with Hoechst 33,258 (blue). Scale bars: 200 μm. b Quantitation of NF-κB p65 nuclear translocation in the indicated groups. c IκBα phosphorylation assessed by western blot analysis. Results are shown as the mean ± SE (n = 3–4). *p < 0.05, **p < 0.01 vs LPS only treatment

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