Fig. 4

The effect of Colla corii asini ethanol extract on cell invasion of S. typhimurium. a Colla corii asini ethanol extract did not have an effect on Caco-2 cell viability. b We treated S. typhimurium with various concentrations of Colla corii asini ethanol extract. After 1 h, Caco-2 cells were washed three times with PBS, and gentamicin (100 μg/ml) was added for 1 h to kill any remaining bacteria. Caco-2 cell lysates were spread on solid agar. After 24 h, we counted the colony numbers. Colla corii asini ethanol extract (0.195 mg/ml) significantly inhibited S. typhimurium invasion in Caco-2 cells