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Fig. 3 | BMC Complementary and Alternative Medicine

Fig. 3

From: The inhibitory effects of compound Muniziqi granule against B16 cells and harmine induced autophagy and apoptosis by inhibiting Akt/mTOR pathway

Fig. 3

Harmine induced autophagy in B16 cells. a: Electron micrographs of normal B16 cells (Control: magnification × 8200) and harmine-treated (40 μM) B16 cells (magnification × 9900); b: Fluorescence microscope (magnification × 200) observation on accumulation of MDC-stained autophagic vacuoles of control and harmine-treated (80 μM) B16 cells; c: The quantitative analysis of MDC-stained cells of harmine treated (40, 50 60 μM) expressed as the mean ± SD (n = 3). *** P < 0.001 vs. the control group; and d: the autophagy induction evaluated by western blot analysis for LC3-II treated with harmine (20, 40, 60, 80 μM) for 24 h and P62 proteins treated with harmine (20, 40, 60 μM) for 2 h

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