Table 1 Program of stressors and duration applied every day. During all these days the saline solution, plant extract or imipramine were administrated depending on the group
Day | Stressors | Duration |
|---|---|---|
1 | Water deprivation | 24 h |
2 | Food deprivation | 24 h |
3–4 | Isolation | 48 h |
5 | Flashing light | 3 h |
6 | Food deprivation | 24 h |
7 | Forced swimming 20 °C | 10 min |
8 | Wet litter | 1 h |
9 | Water deprivation | 24 h |
10 | no stress | – |
11 | Wet litter 4 °C | 2 h |
12 | Flashing light | 1 h 30 min |
13 | Food deprivation | 24 h |
14 | Forced swimming 20 °C | 15 min |
15–16 | Isolation | 48 h |
17 | Water deprivation | 24 h |
18 | Food deprivation | 24 h |
19 | Flashing light | 2 h |
20 | Wet litter | 1 h |
21–22 | Isolation | 48 h |
23 | no stress | – |
24 | Forced swimming 20 °C | 10 min |
25 | Wet litter 4 °C | 2 h |
26 | Food deprivation | 24 h |
27 | Flashing light | 2 h |
28 | Isolation | 24 h |
29 | Water deprivation | 24 h |
30 | Wet litter | 1 h |