Fig. 4

Effect of the ZJW extracts treatment on cell migration and invasion. a Migration assay of SW403 cells treated with ZJW-M, ZJW-H, GR (5 μmol/L) for 18 h. The underside of the filer was stained by crystal violet and observed through microscope (200×). b Migrated cells were counted under a microscope at a magnification of 200× (5 fields/filter). c Invasion assay of SW403 cells treated with ZJW-M, ZJW-H, GR (5 μmol/L) for 18 h. d Cells passed through the Matrigel and filer were counted under a microscope at a magnification of 200× (5 fields/filter). e Representative western blots of MMP2, MMP7, ICAM-1 and CXCR4 in SW403 cells treated with GR (5 μmol/L), ZJW-L, ZJW-M or ZJW-H. f The corresponding semi-quantification data of (e). All values are shown as mean ± SD of three separate experiments, and significant values are indicated with asterisks (*P < 0.05, **P < 0.01 vs. control)