Fig. 2

Effect of the ZJW extracts treatment on SW403 cell proliferation. a Cell proliferation was determined using the CCK8 assay. The total viability of cells treated with ZJW (25, 50, 100, 200, 400 and 800 μg/mL) for 24, 48 and 72 h were measured. b and c The percentage of cells at G1, S, G2 phase after treatment with GR (5 μmol/L), ZJW-M or ZJW-H for 24 h. d Representative western blots of CDK4, cyclin D1 and c-Myc in SW403 cells treated with GR (5 μmol/L), ZJW-L, ZJW-M or ZJW-H. e The corresponding semi-quantification data of d. All values are shown as mean ± SD of three separate experiments, and significant values are indicated with asterisks (*P < 0.05, **P < 0.01 vs. control)