Fig. 2

Effect of CLSE on the migratory ability of HCT116 cells under hypoxic conditions. a. Representative scratch-wound images showing the effect of CLSE on the healing ability of HCT116 cells (magnification: ×40). After with treatment DFO (100 μM) and CLSE (0.5, 1 mg/mL) for 24 h, the migratory ability was analyzed by scratch-wound healing assay. b. Percentage of HCT116 cells that migrated into the wound following CLSE treatment relative to untreated control cells. * p < 0.05 versus untreated control group. ^# p < 0.05 versus DFO-only group. c. Representative images showing the effect of CLSE on HCT116 cell migration through a Transwell® chamber membrane (magnification: ×200). After treatment with CLSE (0.5, 1 mg/mL) for 24 h, the migration cells in transwells were stained by crystal violet solution. d. Percentage of HCT116 cells that migrated following CLSE treatment relative to control cells (normoxia). ** p < 0.01 versus normoxia control group. ^## p < 0.01 versus hypoxia-only group. Data are mean ± SD (n = 3). CLSE, Coix lacryma-jobi var. ma-yuen Strapt sprout extract; H, hypoxia; SD, Standard Deviation