Fig. 5

WERC inhibits c-Fos and NFATc1 expression in osteoclasts. BMMs were incubated with vehicle or WERC (80 μg/mL) for 4 days in the presence of M-CSF and RANKL. (a) Total cell lysate collected for each time point was subjected to western blot analysis with c-Fos or NFATc1 antibody. (b) mRNA levels of Id2, MafB, TRAP, cathepsin K, ATPv0d2, and DC-STAMP were analyzed by qRT-PCR on day 3. (c) BMMs pre-treated with vehicle or WERC (80 μg/mL) in the presence of M-CSF for 3 h were incubated with RANKL (100 ng/mL) for 0, 5, 15, and 30 min. Total cell lysate (30 μg) was subjected to western blot analysis with antibodies specific to JNK, p38, p65, and I-κB. ***p < 0.001 vs. vehicle