Fig. 1
From: Is cytotoxicity a determinant of the different in vitro and in vivo effects of bioactives?
Cytotoxic effects of DHA after 24 (a, b) and 48 (c) hours of supplementation. Cell viability by MTT and AB is expressed as percentage of control cells (assigned as 100%). TB is expressed as percentage of total cells. Cell count is expressed as number of cells per well. Colony count by clonogenic assay is expressed as survival fraction. BAX/BCL2 gene expression ratio is expressed as the mean fold change of relative expression compared to control cells, normalized to one. Data are means ± SD of six samples obtained from two independent experiments. Statistical analysis was by the one-way ANOVA (panel a: MTT and cell count p < 0.001, TB p < 0.05, AB n.s.; panel b: colony count n.s., BAX/BCL2 ratio n.s.; panel c: MTT, TB, and AB p < 0.05, cell count n.s.) using Dunnett’s as post-test to compare supplemented cells to control ones (# p < 0.05; ° p < 0.01; * p < 0.001)