Fig. 2

The effect of DKC-E70 on the expression of cyclin D1 at the protein and mRNA level in human colorectal cancer cells. The cells were plated overnight and then treated with DKC-E70 at the indicated concentrations for 24 h. For Western blot analysis (a), cell lysates were subjected to SDS-PAGE and the Western blot was performed using antibody against cyclin D1. Actin was used as internal control for Western blot analysis. For RT-PCR analysis of the gene expression of cyclin D1 (b), total RNA was prepared. GAPDH was used as internal control for RP-PCR. (c) HCT116 cells were treated with DKC-E70 (50 μg/ml) for the indicated times. The protein and mRNA level of cyclin D1 was analyzed using Western blot and RT-PCR, respectively. *P < 0.05 compared to cell without DKC-E70 treatment