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Table 3 Cytotoxicity results of T cordifolia on HeLa cell line. Cytotoxicity results of T cordifolia by MTT assay on HeLa (Human cervical carcinoma)

From: Assessment of free radical scavenging and anti-proliferative activities of Tinospora cordifolia Miers (Willd)

S.No

Concentration (μg/mL)

% Cell Death

TCE

TCP

TCD

TCB

TCA

Doxorubicin

1

0.01

–

–

–

–

–

39.81 ± 1.06**

2

0.1

–

–

–

–

–

48.24 ± 0.27**

3

1

–

–

–

–

–

68.43 ± 1.02**

4

10

–

–

–

–

–

82.54 ± 0.92**

5

25

39.92 ± 0.36*

29.24 ± 0.64

55.02 ± 0.68**

3.63 ± 1.54

13.47 ± 1.88

–

6

50

56.06 ± 0.73**

53.34 ± 0.48**

55.95 ± 0.43**

29.79 ± 1.79

25.84 ± 1.51

–

7

100

66.76 ± 0.51**

66.00 ± 1.12*

73.90 ± 0.59**

40.95 ± 0.69*

28.58 ± 1.98

–

8

200

80.97 ± 1.71**

77.30 ± 0.33*

81.60 ± 0.28**

76.54 ± 0.58*

84.02 ± 0.46**

–

9

IC50

101.26 ± 1.42b

131.94 ± 1.23c

54.23 ± 0.94a

276.03 ± 2.00

366.05 ± 1.74

1.89 ± 0.02a

  1. Results were expressed as Mean ± SEM (n = 3) and analysed using one-way ANOVA followed by Tukey’s post hoc test of significance [where different alphabets denote significant difference (p < 0.05)]. Statistical comparison between the mean % viability of each extract and its negative control was performed using one-way ANOVA followed by Dunnett’s post hoc test of significance wherein *p < 0.05 and **p < 0.01 were considered to be statistically significant and very statistically significant compared to negative control. T cordifolia extracts were tested at concentrations of 200, 100, 50, 25 μg/mL and Doxorubicin was tested at concentrations of 0.01, 0.1, 1 and 10 μg/mL