Fig. 2

DCs are dispensable for TWE-mediated T cell polarization. a Either TWE or vehicle was supplemented in the drinking water of yeti (IFNγ/YFP), 4get (IL4/GFP), and Foxp3 (GFP) reporter mice for 5 days. Subsequently, the level of reporter protein expression was assessed in CD4⁺ T cells (CD3⁺CD4⁺), CTLs (CD3⁺CD8⁺), and Treg cells (CD3⁺CD4⁺CD25⁺) using flow cytometry. The mean values ± SD (n = 4, * P < 0.05, ** P < 0.01) are shown. b Either TWE or vehicle was supplemented in the drinking water of DT (120 ng/mouse)-treated CD11c-DTR tg mice. Three days later, DT was intraperitoneally reinjected for the further depletion of DCs. Two days after the second DT injection, the mice were sacrificed and subsequently single cells were prepared from the spleen and MLN. Intracellular IFNγ, IL4, and Foxp3 productions were assessed in CD4⁺ T cells (CD3⁺CD4⁺) and Treg cells (CD3⁺CD4⁺CD25⁺) by flow cytometry. The data are representative of two independent experiments (n = 4, * P < 0.05, ** P < 0.01)