Fig. 5From: Inhibition of human cytomegalovirus immediate-early gene expression and replication by the ethyl acetate (EtOAc) fraction of Elaeocarpus sylvestris in vitroDownregulation of HCMV lytic gene expression by the EtOAc fraction of ESE. HFF cells were infected with HCMV-Towne at an MOI of 1 and treated with either DMSO or the EtOAC fraction of ESE (50 μg/ml). At 0, 24, 48 or 72 h after infection, equal amounts of cell extracts were subjected to western blot analysis with antibodies against HCMV IE (IE1-72 kDa and IE2-86 kDa), ICP 36, UL83 and tubulinBack to article page