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Fig. 6 | BMC Complementary and Alternative Medicine

Fig. 6

From: The ethanol extract of Aquilariae Lignum ameliorates hippocampal oxidative stress in a repeated restraint stress mouse model

Fig. 6

NF-kappa B (NF-κB) activation in lipopolysaccharide (LPS)-treated BV2 microglial cells. Cells were pre-treated with vehicle, ALE (1 and 5 μg/mL) or NAC (10 μM) for 3 h before 1 h exposure to LPS (1 μg/mL). Immunofluroscent staining shows the cellular distribution of NF-κB p65 subunit (green) in microglia. The nuclei were counterstained with DAPI (blue) (a). The p65 expressions in cytosolic and nucleic extracts, respectively, were determined by western blot and semi-quantified using Image J (b, c). The data are obtained from three independent experiments and expressed as the means ± SD (n = 3). ## P < 0.01 compared with control group; * P < 0.05, ** P < 0.01 compared with LPS group

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