Fig. 3

Effects of 7-methoxycoumarin (MC) on cell proliferation and on estrogen receptors. MCF-7 cells proliferation (a); activation of estrogen α (b) and β (c) receptors in HEK293T cells. The relative MCF-7 cells yields (PE) were measured in the presence of DMSO (0.01%), 17β-estradiol (10 nM) and MC. The effect of 7-methoxycoumarin on estrogen α and β receptors activity in the transiently transfected HEK293T-ERα and HEK293T-ERβ cells was investigated by measuring reporter gene-coupled luciferase activity. The relative luciferase units (RLU) were measured in the presence of EtOH (0.1%), E2 (10 nM), 7-methoxycoumarin (MC) co-treated with E2 (a & b). PE = max cell number of sample/cell number of DMSO control. *** p < 0.001 as compared to EtOH control; ### p < 0.001 as compared with E2 control. ^ap < 0.05, ^cp < 0.001 as compared to DMSO control