Table 2 Primers and quantitative PCR conditions to determine expression of murine immunomodulatory cytokines
From: Aqueous extract of Phragmitis rhizoma ameliorates myelotoxicity of docetaxel in vitro and in vivo
Target | Forward (5′ → 3′) | Reverse (5′ → 3′) | GenBank Accession # |
 GAPDH | TTGATGGCAACAATCTCCAC | CGTCCCGTAGACAAAATGGT | NM_001001303 |
 GM-CSF | CCGTAGACCCTGCTCGAATA | TGCCTGTCACATTGAATGAA | NM_009969.4 |
 IFNγ | ATGAACGCTACACACTGCATC | CCATCCTTTTGCCAGTTCCTC | NM_008337 |
 IL1β | GCAACTGTTCCTGAACTCAACT | ATCTTTTGGGGTCCGTCAACT | NM_008361 |
 IL3 | All-in-One™ qPCR primer (MQP029461) | NM_010556.4 | |
 IL6 | TAGTCCTTCCTACCCCAATTTCC | TTGGTCCTTAGCCACTCCTTC | NM_031168 |
 IL7 | TTCCTCCACTGATCCTTGTTCT | AGCAGCTTCCTTTGTATCATCAC | X07962.1 |
 IL12β | TGGTTTGCCATCGTTTTGCTG | ACAGGTGAGGTTCACTGTTTCT | NM_008352 |
 SCF | ATGTTCCCCGCTCTCTTTGG | GTGTGGCATAAGGGCTCACT | NM_013598.2 |
 TNFα | CCCTCACACTCAGATCATCTTCT | GCTACGACGTGGGCTACAG | NM_013693 |
Step | Temperature (°C) | Time (s) | # of cycle |
 Activation | 95 | 30 | 1 |
 Amplification | 95 | 15 | 40 |
60 | 30 | ||
 Melting-curve | 65–95 | 2 s/step | 1 |