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Fig. 5 | BMC Complementary and Alternative Medicine

Fig. 5

From: Moringa isothiocyanate complexed with α-cyclodextrin: a new perspective in neuroblastoma treatment

Fig. 5

MAC induces apoptosis pathway in SH-SY5Y cells. Western blot analysis for cleaved caspase-3 (a). CTR vs 1.0 μg ** p = 0,0056; CTR vs 5.0 μg **** p < 0.0001; 1.0 μg vs 2.5 μg ** p = 0.0022; 1.0 μg vs 5.0 μg **** p < 0.0001; 2.5 μg vs 5.0 μg **** p < 0.0001. Western blot analysis for Bax (b). CTR vs 5.0 μg **** p < 0.0001; 1.0 μg vs 5.0 μg **** p < 0.0001; 2.5 μg vs 5.0 μg **** p < 0.0001. Western blot analysis for Bcl-2 (c). CTR vs 5.0 μg **** p < 0.0001; 1.0 μg vs 2.5 μg * p = 0.0006; 1.0 μg vs 5.0 μg **** p < 0.0001; 2.5 μg vs 5.0 μg **** p < 0.0001. Western blot analysis for p53 (d). CTR vs 1.0 μg ** p = 0,0032; CTR vs 2.5 μg *** p = 0,0001; CTR vs 5.0 μg ** p = 0,0092; 1.0 μg vs 5.0 μg **** p < 0.0001; 2.5 μg vs 5.0 μg **** p < 0.0001. Western blot analysis for p21 (e). CTR vs 2.5 μg ** p = 0,0129; CTR vs 5.0 μg **** p < 0.0001; 1.0 μg vs 2.5 μg * p = 0.0129; 1.0 μg vs 5.0 μg **** p < 0.0001; 2.5 μg vs 5.0 μg **** p < 0.0001. Results were analyzed by one-way ANOVA followed by a Bonferroni test for multiple comparisons. Blots are representative of three separate and reproducible experiments. The statistical analysis was carried out on three repeated blots performed on separate experiments

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