Fig. 3

Inhibition of PI3K/Akt/mTOR and MAPK pathways in response to MAC in SH-SY5Y cells. Western blot analysis for pPI3K (a). CTR vs 1.0 μg ^**p = 0.0017; CTR vs 2.5 μg ^***p = 0,0001; CTR vs 5.0 μg ^****p < 0.0001; 1.0 μg vs 5.0 μg ^*p = 0,0409. Western blot for pAKT (b). CTR vs 5.0 μg ^**p = 0,0011; 1.0 μg vs 5.0 μg ^**p = 0,0021; 2.5 μg vs 5.0 μg ^**p = 0,0019. Western blot for pmTOR (c) CTR vs 2.5 μg ^**p = 0.0017; CTR vs 5.0 μg ^****p < 0.0001; 1.0 μg vs 2.5 μg ^***p = 0,0005; 1.0 μg vs 5.0 μg ^****p < 0.0001; 2.5 μg vs 5.0 μg ^***p = 0,0002. Western blot analysis for pJNK (d). CTR vs 1.0 μg ^***p = 0.0001; CTR vs 2.5 μg ^****p < 0.0001; CTR vs 5.0 μg ^****p < 0.0001; 1.0 μg vs 2.5 μg ^*p = 0,0122; 1.0 μg vs 5.0 μg ^*p = 0,0242. Western blot analysis for pp38 (E). CTR vs 1.0 μg ^****p < 0.0001; CTR vs 2.5 μg ^****p < 0.0001; CTR vs 5.0 μg ^****p < 0.0001; 1.0 μg vs 5.0 μg ^****p < 0.0001; 2.5 μg vs 5.0 μg ^****p < 0.0001. Results were analyzed by one-way ANOVA followed by a Bonferroni test for multiple comparisons. Blots are representative of three separate and reproducible experiments. The statistical analysis was carried out on three repeated blots performed on separate experiments