Fig. 2

Anti-proliferative effects of MAC on SH-SY5Y cell line. Cells were cultured in the presence of increased concentrations of MAC for 24, 48 and 72 h. Proliferation rates of SH-SY5Y cells were evaluated by MTT. 5-FU was considered the positive standard (a). Results of MTT are expressed as percentage ± SEM of absorbance detected in treated cells. The MTT data were confirmed by the analysis of the growth curve obtained by counting the cells in a Neubauer hemocytometer chamber after MAC administration for 24, 48 and 72 h (b). The cytotoxic activities of MAC exposure for 24 h was evaluated in terms of both LDH release (c) and cell death (d). LDH levels are extrapolated as the values detected in control cells which are arbitrarily expressed as 1. Cell death was reported as the percentage of blue stained (non-viable) vs total cells counted. Data, expressed as mean ± SEM, represent the values obtained in three different sets of experiments made in triplicate. . ^*p = 0.01 vs CTR; ^**p = 0.001; ^****p < 0.0001 vs CTR