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Fig. 5 | BMC Complementary and Alternative Medicine

Fig. 5

From: Anti-diabetic activity of aerial parts of Sarcopoterium spinosum

Fig. 5

Glucose uptake induced by aerial parts of S. spinosum is mediated by PI3K and Akt. Differentiated 3 T3-L1 adipocytes were treated with leaves and fruits (2 mg/ml) or roots (1 mg/ml) extracts, with the use of insulin (100 nM) as a positive control, in the presence or absence of LY294002 (a), AKT inhibitor VIII (b), compound C (c), or PD 98059 (d). The uptake of [3H]2-Deoxy-D-glucose into cells was determined as described in Materials and Methods. Data are expressed as percent of basal uptake in control cells. The data represents the mean ± SE of measurement made on three replicates in each of at least 4 independent experiments. #p < 0.05, ##p < 0.005, ### p < 0.0005 compared to untreated cells, **p < 0.005, *** p < 0.0005 comparing cells treated with the indicated extract in the presence or absence of the indicated inhibitor, analyzed by one-way analysis of variance (ANOVA), followed by Bonferroni’s post-hoc testing

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