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Table 1 Sequences of oligonucleotide primers and details of polymerase chain reactions

From: Xue-fu-Zhu-Yu decoction protects rats against retinal ischemia by downregulation of HIF-1α and VEGF via inhibition of RBP2 and PKM2

mRNA

Primers (5’→3’)

F: Forward

R: Reverse

Bases in base pairs

Product size

Cycles profile

Denaturation/annealing/extension

(Temperature and time in seconds)

Cycles number

β-actin

F: AGGGAAATCGTGCGTGACAT

694 713

150

95°C / 95°C / 60°C

40

R: GAACCGCTCATTGCCGATAG

824-843

 

(20s / 3s / 30s)

VEGF

F: GCGGGCTGCTGCAATG

1262-1277

268

95°C / 95°C / 60°C

40

R: TGCAACGCGAGTCTGTGTTT

1528-1547

 

(20s / 3s / 30s)

HIF-1α

F: ACAGCTCCCCAGCATTTCAC

2748-2767

90

95°C / 95°C / 60°C

40

R: GGACAAACTCCCTCACCAAAAA

2837-2816

 

(20s / 3s / 30s)

PKM2

F: TCTACGTGGACGATGGGCT

833-851

403

95°C / 95°C / 60°C

40

R: AGGAAGACCTTCTCTGCCGGA

1215-1235

 

(20s / 3s / 30s)

RBP2

F: TTGTGGTGACGTTTCCTCGT

2093-2112

213

95°C / 95°C / 60°C

40

R: CAGCCAGCCCCACATCTAAG

2305-2286

 

(20s / 3s / 30s)