Fig. 3
Cresyl violet staining: analysis of the thickness of various retinal layers. Sectioned retinal pieces of the identical eccentricity are shown. a is from a retina subjected to the sham procedure (Sham). b is from a retina that was given ischemia plus reperfusion (I/R) and preischemia administration of vehicle. c, d, e are sections from retinas that have received ischemia plus reperfusion and preischemia administration of 1.35 g/kg/day XFZYD (c, XFZYD1.35 + I/R), 2.7 g/kg/day XFZYD (d, XFZYD2.7 + I/R) or postischemia administration with 2.7 g/kg/day XFZYD (e, I/R + XFZYD2.7). The thickness of the different retinal layers from sectioned retinal pieces of the identical eccentricity is demonstrated. f The results indicate the mean values ± SD (n = 4). ** indicates a significant difference (p < 0.01) from the Sham retina. † or †† indicates a significant difference (p < 0.05 or p < 0.01) from the Vehicle + I/R retina. Abbreviations: XFZYD, Xue-Fu-Zhu-Yu decoction; ONL, outer nuclear layer; OPL, outer plexiform layer; INL, inner nuclear layer; IPL, inner plexiform layer; GCL, ganglion cell layer. Scale bar = 50 μm