Fig. 8
From: In vitro induction of human embryonal carcinoma differentiation by a crude extract of Rhazya stricta
RT-PCR analysis of stem cell genes in NT2 cells after treatment for 14 days with RS1S CHCL3 extract (5 μg/ml). Representative agarose gel electrophoresis patterns of RT-PCR products showed reduced expression of Oct4 and Sox2. All gene expression data were normalized to β-actin as an internal control. Band intensity was measured and the values were expressed as the mean ± SD of two independent experiments. *P < 0.05 compared to vehicle control (VC; 0.001% MeOH)