Fig. 5

The effect of LTAP-JO on DNCB-mediated molecular and biological changes in the skin lesion. (a) The levels of IgE, TARC, and IFNγ in the dorsal skin tissues of mice were measured using an ELISA kit. Data are shown as the relative level of the proteins, and represents the mean ± SEM (n = 5). Means with the symbols (*, **) indicate significant differences (p < 0.05). (b) The result of the RT-PCR and Western Blot assay using the skin tissues. Data shown are representatives of 4 independent experiments. (c) The location of NFκB in the skin tissue was monitored by an immunofluorescence assay against NFκB. Photographs were taken under a confocal microscope at 400× magnification. Data shown are the representatives of each group of mice