Fig. 2

The individual (a) and synergetic (b) effect of fenugreek seed (FS), mulberry leaf (ML) and American ginseng (AG) extracts on insulin sensitivity in differentiated primary human subcutaneous adipocytes. After 20 h of pre-treatment with extracts or botanical test formula, glucose uptake was initiated with the addition of a cocktail containing 2-DOG and ³H-2-DOG with 1 nM insulin and incubate for 2 h. Change in insulin sensitivity was determined by setting 1 nM insulin as 0% and 100 nM as 100%, and calculating the percent change in the delta between 1 nM and 100 nM insulin. Data are presented as mean ± SD of triplicates. ^* P < 0.05 vs. 1:1:1 ratio group