Cell cycle arrest and apoptosis induction by methanolic leaves extracts of four Annonaceae plants

Pumiputavon, Kitti; Chaowasku, Tanawat; Saenjum, Chalermpong; Osathanunkul, Maslin; Wungsintaweekul, Boonsong; Chawansuntati, Kriangkrai; Wipasa, Jiraprapa; Lithanatudom, Pathrapol

doi:10.1186/s12906-017-1811-3

BMC Complementary Medicine and Therapies

Table 1 The average (±SD) percentages of apoptotic cells identified from the dot plots of Annexin V vs PI with statistical analysis

From: Cell cycle arrest and apoptosis induction by methanolic leaves extracts of four Annonaceae plants

	Concentrations of crude extracts (μg/ml)				Controls
	1000	500	250	125	UT-C^a	DMSO-C^b
HeLa
LUL^c	50.9 ± 4.8**	45.3 ± 1.7**	37.3 ± 3.7**	16.2 ± 6.8**	3.1 ± 1.5	3.2 ± 1.3
LD^d	22.8 ± 1.6**	22.9 ± 1.7**	22.1 ± 0.0**	17.9 ± 0.5**
LAB^e	13.4 ± 2.1**	6.0 ± 1.0**	5.9 ± 2.7*	6.6 ± 1.9**
LMM^f	70.2 ± 5.0**	33.7 ± 6.9**	2.8 ± 0.4	2.1 ± 0.1
SiHa
LUL^c	17.0 ± 1.3**	30.0 ± 2.0**	18.8 ± 1.3**	7.9 ± 0.0*	5.2 ± 1.7	8.6 ± 1.4
LD^d	93.1 ± 1.3**	77.1 ± 2.1**	19.2 ± 0.1**	3.2 ± 0.4**
LAB^e	87.9 ± 4.8**	84.5 ± 3.7**	37.2 ± 6.2**	4.5 ± 0.4**
LMM^f	96.6 ± 0.1**	15.3 ± 1.5**	3.5 ± 1.1**	3.9 ± 0.2**
CaSki
LUL^c	37.5 ± 1.5**	16.1 ± 3.7**	20.6 ± 0.4**	6.0 ± 0.8	6.0 ± 0.2	6.4 ± 1.4
LD^d	88.2 ± 1.8**	77.9 ± 2.2**	51.9 ± 4.4**	7.6 ± 0.0
LAB^e	89.5 ± 4.6**	51.9 ± 3.1**	38.0 ± 1.0**	3.9 ± 0.6**
LMM^f	76.1 ± 2.6**	34.4 ± 0.8**	5.6 ± 0.4	7.0 ± 0.9
HepG2
LUL^c	87.1 ± 4.7**	65.2 ± 1.1**	36.7 ± 2.2**	38.9 ± 2.4**	11.1 ± 6.6	12.4 ± 6.6
LD^d	84.6 ± 3.8**	75.1 ± 1.9**	45.0 ± 0.3**	42.8 ± 3.8**
LAB^e	80.1 ± 2.4**	70.5 ± 4.6**	76.7 ± 0.4**	16.4 ± 0.4
LMM^f	93.7 ± 2.1**	56.5 ± 4.0**	46.1 ± 7.3**	40.2 ± 2.6**
Hep3B
LUL^c	51.1 ± 0.1**	27.3 ± 2.0**	12.0 ± 0.9**	10.5 ± 1.0**	8.1 ± 0.6	6.9 ± 0.9
LD^d	43.1 ± 2.5**	13.3 ± 7.9*	7.5 ± 2.6	5.9 ± 0.3
LAB^e	54.2 ± 2.3**	57.6 ± 3.6**	43.0 ± 1.3**	36.8 ± 1.3**
LMM^f	45.6 ± 6.9**	14.0 ± 2.2**	7.0 ± 8.6	9.1 ± 8.4
K562
LUL^c	44.2 ± 0.7**	8.2 ± 0.0**	6.7 ± 1.1	6.8 ± 0.9	5.6 ± 1.3	5.0 ± 0.1
LD^d	25.9 ± 4.2**	7.4 ± 0.7**	7.5 ± 2.1	5.6 ± 0.1
LAB^e	77.1 ± 6.6**	37.3 ± 3.2**	22.9 ± 1.7**	7.3 ± 1.6
LMM^f	79.6 ± 1.3**	13.5 ± 1.3**	5.7 ± 0.6	5.5 ± 0.7
U937
LUL^c	94.2 ± 0.9**	10.1 ± 0.0**	6.0 ± 1.7	6.2 ± 0.0	7.4 ± 1.4	5.7 ± 0.7
LD^d	88.8 ± 0.3**	8.8 ± 0.4*	8.0 ± 0.3	7.4 ± 1.2
LAB^e	29.1 ± 4.1**	8.6 ± 4.2*	5.4 ± 0.9	6.2 ± 0.2
LMM^f	84.6 ± 3.0**	54.9 ± 1.2**	5.3 ± 4.9	5.0 ± 3.7
RAJI
LUL^c	71.8 ± 3.4**	32.6 ± 1.6**	29.9 ± 2.6**	23.8 ± 0.5**	5.6 ± 2.2	7.0 ± 3.1
LD^d	81.0 ± 2.9**	26.9 ± 0.3**	21.1 ± 0.2**	19.4 ± 2.2**
LAB^e	98.6 ± 0.1**	93.3 ± 0.6**	41.1 ± 0.4**	24.5 ± 6.1**
LMM^f	98.5 ± 0.3**	88.5 ± 3.0**	33.7 ± 1.0**	25.3 ± 2.4**

Symbols * and ** represent statistically significant differences less than 0.05 and 0.01, respectively, when compared to DMSO-treated control
^aUntreated control
^bDMSO-treated control
^cCrude extract from leaf of U. longipes
^dCrude extract from leaf of Dasymaschalon sp
^eCrude extract from leaf of A. burmanicus
^fCrude extract from leaf of M. modestum

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