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Table 1 Composition of mobile phase with gradient elution

From: Anti-obesity activity, acute toxicity, and chemical constituents of aqueous and ethanol Viola mandshurica extracts

Time (Min) A% (0.1% Trifluoroacetic Acid in Water) B% (Actonitrile)
0–10 90 10
10 90 10
50 50 50
52 0 100
58 0 100
70 90 10
70 90 10