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Fig. 7 | BMC Complementary and Alternative Medicine

Fig. 7

From: Inhibition of biofilm formation in Mycobacterium smegmatis by Parinari curatellifolia leaf extracts

Fig. 7

The effect of combining P. curatellifolia ethanol extract with kanamycin. a M. smegmatis was incubated in a medium containing 1.6 μg/ml kanamycin and varying ethanol extract concentration for 72 h. The amount of biofilm that had formed was determined by staining the biofilms with crystal violet, dissolving the crystal violet on the stained biofilms and spectrophotometrically measuring the amount of dye. b M. smegmatis was incubated for 72 h in a medium containing 100 μg/ml ethanol extract and varying concentration of kanamycin from MIC value to quarter MIC value. The Error bars indicates the standard deviation from mean (n = 4). Dunnet’s multiple comparison test was used to compare columns using graph pad prism 5 software. The asterisks (*) indicate statistical significant differences with the growth control (media + cells) * < 0.05 *** < 0.0001

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