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Table 8 Anti-Pharaoh ant effect of various samples of Isodon rugosus

From: Demonstration of biological activities of extracts from Isodon rugosus Wall. Ex Benth: Separation and identification of bioactive phytoconstituents by GC-MS analysis in the ethyl acetate extract

Samples Dose (mg/ml) Total treated No. repeated Mortality(%) mean ± SEM LD50 (mg/ml)
Ir.Cr 0.5 20 3 41.33 ± 0.88*** 0.85
1 20 52.00 ± 0.57***
2 20 57.67 ± 0.67***
4 20 58.00 ± 0.57***
6 20 70.00 ± 0.00***
8 20 81.33 ± 0.88***
Ir.Hex 0.5 20 3 39.67 ± 1.20*** 1.13
1 20 49.00 ± 1.15***
2 20 53.00 ± 0.57***
4 20 56.67 ± 0.67***
6 20 58.00 ± 0.57***
8 20 80.67 ± 1.20***
Ir.Cf 0.5 20 3 67.33 ± 1.45*** <0.1
1 20 68.67 ± 0.67***
2 20 73.33 ± 0.88***
4 20 76.00 ± 1.15***
6 20 85.00 ± 0.57**
8 20 95.33 ± 0.88ns
Ir.EtAc 0.5 20 3 72.00 ± 1.15 *** <0.1
1 20 77.67 ± 0.67 ***
2 20 80.33 ± 0.33**
4 20 86.33 ± 0.88*
6 20 90.67 ± 0.67ns
8 20 100.0 ± 0.57ns
Ir.Aq 0.5 20 3 29.00 ± 1.15*** 3.37
1 20 41.33 ± 0.88 ***
2 20 44.67 ± 1.20***
4 20 53.33 ± 0.33 ***
6 20 66.00 ± 0.57***
8 20 80.67 ± 1.76***
Ir.Sp 0.5 20 3 96.33 ± 0.88ns <0.1
1 20 100.0 ± 0.00ns
2 20 100.0 ± 0.00ns
4 20 100.0 ± 0.00ns
6 20 100.0 ± 0.00ns
8 20 100.0 ± 0.00ns
Permithrin 0.5 20 3 93.33 ± 4.40 <0.1
1 20 98.33 ± 1.66
2 20 100.0 ± 0.00
4 20 100.0 ± 0.00
6 20 100.0 ± 0.00
8 20 100.0 ± 0.00
Distilled Water Negative control 20 3 0.00 0.00
  1. Data is represented as mean ± SEM; (n = 3). Values significantly different (*: P < 0.05, ** P < 0.01 and *** P < 0.001) as compared to the control drug
  2. Keys:
  3. Ir.Cr Crude methanolic extract, Ir.Hex n-hexane fraction, Ir.Cf Chloroform fraction, Ir.EtAc Ethyl acetate fraction, Ir.Aq Aqueous fraction, Ir.Sp Saponins.