Demonstration of biological activities of extracts from Isodon rugosus Wall. Ex Benth: Separation and identification of bioactive phytoconstituents by GC-MS analysis in the ethyl acetate extract

Table 8 Anti-Pharaoh ant effect of various samples of Isodon rugosus

Samples	Dose (mg/ml)	Total treated	No. repeated	Mortality(%) mean ± SEM	LD₅₀ (mg/ml)
Ir.Cr	0.5	20	3	41.33 ± 0.88^***	0.85
	1	20		52.00 ± 0.57^***
	2	20		57.67 ± 0.67^***
	4	20		58.00 ± 0.57^***
	6	20		70.00 ± 0.00^***
	8	20		81.33 ± 0.88^***
Ir.Hex	0.5	20	3	39.67 ± 1.20^***	1.13
	1	20		49.00 ± 1.15^***
	2	20		53.00 ± 0.57^***
	4	20		56.67 ± 0.67^***
	6	20		58.00 ± 0.57^***
	8	20		80.67 ± 1.20^***
Ir.Cf	0.5	20	3	67.33 ± 1.45^***	<0.1
	1	20		68.67 ± 0.67^***
	2	20		73.33 ± 0.88^***
	4	20		76.00 ± 1.15^***
	6	20		85.00 ± 0.57^**
	8	20		95.33 ± 0.88^ns
Ir.EtAc	0.5	20	3	72.00 ± 1.15 ^***	<0.1
	1	20		77.67 ± 0.67 ^***
	2	20		80.33 ± 0.33^**
	4	20		86.33 ± 0.88^*
	6	20		90.67 ± 0.67^ns
	8	20		100.0 ± 0.57^ns
Ir.Aq	0.5	20	3	29.00 ± 1.15^***	3.37
	1	20		41.33 ± 0.88 ^***
	2	20		44.67 ± 1.20^***
	4	20		53.33 ± 0.33 ^***
	6	20		66.00 ± 0.57^***
	8	20		80.67 ± 1.76^***
Ir.Sp	0.5	20	3	96.33 ± 0.88^ns	<0.1
	1	20		100.0 ± 0.00^ns
	2	20		100.0 ± 0.00^ns
	4	20		100.0 ± 0.00^ns
	6	20		100.0 ± 0.00^ns
	8	20		100.0 ± 0.00^ns
Permithrin	0.5	20	3	93.33 ± 4.40	<0.1
	1	20		98.33 ± 1.66
	2	20		100.0 ± 0.00
	4	20		100.0 ± 0.00
	6	20		100.0 ± 0.00
	8	20		100.0 ± 0.00
Distilled Water	Negative control	20	3	0.00	0.00

Data is represented as mean ± SEM; (n = 3). Values significantly different (*: P < 0.05, ** P < 0.01 and *** P < 0.001) as compared to the control drug
Keys:
Ir.Cr Crude methanolic extract, Ir.Hex n-hexane fraction, Ir.Cf Chloroform fraction, Ir.EtAc Ethyl acetate fraction, Ir.Aq Aqueous fraction, Ir.Sp Saponins.

ISSN: 2662-7671