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Fig. 4 | BMC Complementary and Alternative Medicine

Fig. 4

From: Wogonin suppresses stem cell-like traits of CD133 positive osteosarcoma cell via inhibiting matrix metallopeptidase-9 expression

Fig. 4

Effects of Wogonin on the renewal capacity of CD133+ CAL72 CSC. a The effect of Wogonin on inhibiting the number of spheres. b Single sphere formation of CD133+ CAL72 cells after 9 days in suspension culture containing Wogonin (0, 10, 20, 40, and 80 μM). c Wogonin reduced the clonogenicity of CD133+ CAL72 cells; each well was seeded with 1 × 103 cells and incubated at 37 °C with 5% CO2 after 24 h of Wogonin treatment. Then, the colonies were visualized by staining with 0.5% crystal violet. Data are represented as mean ± SEM (n = 3). P < 0.05, P < 0.01, **** P < 0.0001

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