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Fig. 3 | BMC Complementary and Alternative Medicine

Fig. 3

From: Wogonin suppresses stem cell-like traits of CD133 positive osteosarcoma cell via inhibiting matrix metallopeptidase-9 expression

Fig. 3

Wogonin inhibits the invasion potential of CD133+ CAL72 CSC. a Cells were treated with varying concentrations of Wogonin (0, 10, 20, 40, and 80 μM) 24 h prior to the invasion assay, followed by staining with 0.5% crystal violet. b Invading cells were calculated by counting cells in five random fields under the microscope. c Supernatant of the medium containing cultured Wogonin-treated human osteosarcoma CSC was subjected to gelatin zymography assay. d Western blot assay of total extract proteins derived from 24-h treated CSC exposed to varying concentrations of Wogonin (0, 10, 20, 40, and 80 μM). Data are represented as mean ± SEM (n = 3). P < 0.05, P < 0.01, *** P < 0.001, **** P < 0.0001

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