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Table 1 Results of the quantitative fluorescent assay for simultaneous identification of apoptotic and necrotic cells in C. albicans ATCC 90028 treated with berberine in vitro for 3 h

From: Membrane of Candida albicans as a target of berberine

Sample

Viable cells (%)

Non-viable cells

Σ

Apoptosis (%)

Necrosis (%)

½ MIC

88.7 ± 2.1

11.3 ± 2.1MIC,2xMIC,NC,PC

7.7 ± 1.5 MIC,2xMIC,PC

3.7 ± 2.52xMIC,PC

MIC

75.3 ± 2.5

24.7 ± 2.52xMIC,NC,PC

18.0 ± 1.72xMIC,NC,PC

6.7 ± 1.2NC

2× MIC

62.3 ± 9.7

37.7 ± 9.7NC

28.3 ± 10.3NC

9.3 ± 0.6NC

PC

56.0 ± 4.6

44.0 ± 4.6NC

35.3 ± 2.3NC

8.7 ± 2.5NC

NC

94.7 ± 1.2

5.3 ± 1.2

4.0 ± 1.7

1.3 ± 0.6

  1. 300 cells per sample per each experimental point were analysed. Mean values ± SD are shown. MIC minimal inhibitory concentration, PC positive control, NC negative control (RPMI). Statistical significance of data was evaluated using χ2 test. The level of statistical significance was set at P < 0.05. The abbreviations next to the means indicate from which groups the relevant group differs with statistical significance