Skip to main content

Table 1 Results of the quantitative fluorescent assay for simultaneous identification of apoptotic and necrotic cells in C. albicans ATCC 90028 treated with berberine in vitro for 3 h

From: Membrane of Candida albicans as a target of berberine

Sample Viable cells (%) Non-viable cells
Σ Apoptosis (%) Necrosis (%)
½ MIC 88.7 ± 2.1 11.3 ± 2.1MIC,2xMIC,NC,PC 7.7 ± 1.5 MIC,2xMIC,PC 3.7 ± 2.52xMIC,PC
MIC 75.3 ± 2.5 24.7 ± 2.52xMIC,NC,PC 18.0 ± 1.72xMIC,NC,PC 6.7 ± 1.2NC
2× MIC 62.3 ± 9.7 37.7 ± 9.7NC 28.3 ± 10.3NC 9.3 ± 0.6NC
PC 56.0 ± 4.6 44.0 ± 4.6NC 35.3 ± 2.3NC 8.7 ± 2.5NC
NC 94.7 ± 1.2 5.3 ± 1.2 4.0 ± 1.7 1.3 ± 0.6
  1. 300 cells per sample per each experimental point were analysed. Mean values ± SD are shown. MIC minimal inhibitory concentration, PC positive control, NC negative control (RPMI). Statistical significance of data was evaluated using χ2 test. The level of statistical significance was set at P < 0.05. The abbreviations next to the means indicate from which groups the relevant group differs with statistical significance