Fig. 9

Acute effect of eugenol on fasting blood glucose levels in in vivo diabetic rats and glucose-stimulated insulin secretion in in vitro mice isolated islets. a EG was administered orally and blood glucose was measured at 0, 60 and 120 mins. Values are mean ± SD for five rats per group. Control, nondiabetic control rats; Control + EG, control rats treated with EG of 50 mg/kg; Db, diabetic control rats; Db + EG, diabetic rats treated with EG of 50 mg/kg. *P < 0.05 vs. 0-min value (paired t-test). b Groups of 3 size-matched mice isolated islets were incubated for one hour at 37 °C in KRB buffer, containing 3 mM or 16.7 mM glucose in the absence or presence of EG and secreted insulin was measured. Values are mean ± SEM from 3 independent experiments. ^† p < 0.05 compared with the value for 3 mM glucose alone; *p < 0.05, **p < 0.01 compared with the value for 16.7 mM glucose alone (one-way ANOVA with Dunnett’s multicomparison test). TB, tolbutamide (200 μM)