Skip to main content

Table 1 The IC50 values of LAMF, LAMR, morin, and chlorogenic acid in various antioxidant assays (μg/mL or μM)

From: Lyophilized aqueous extracts of Mori Fructus and Mori Ramulus protect Mesenchymal stem cells from •OH–treated damage: bioassay and antioxidant mechanism

Assays

LAMF

LAMR

morin

chlorogenic acid

luteolin

Trolox

PTIO• Scavenging

6609.7 ± 756.6d

4286.9 ± 84.9c

103.4 ± 0.9b (344.6 ± 2.9C)

99.6 ± 2.3b

(281.4 ± 6.5B)

ND

40.6 ± 6.1a (162.2 ± 24.2 A)

DPPH•-scavenging

208.7 ± 3.0d

97.3 ± 3.1c

8.2 ± 0.7b

(27.1 ± 2.2 D)

5.6 ± 0.01a

(15.8 ± 0.03B)

3.2 ± 0.1a

(11.1 ± 0.2A)

4.5 ± 0.1a

(18.1 ± 0.4 C)

ABTS• scavenging

73.5 ± 5.8c

34.4 ± 0.1b

4.2 ± 0.2a

(13.9 ± 0.6 B)

5.8 ± 0.3a

(16.4 ± 0.9 B)

2.6 ± 0.1a

(9.0 ± 0.2A)

1.9 ± 0.6a

(7.7 ± 2.6A)

FRAP

277.0 ± 3.1c

191.9 ± 5.2b

5.0 ± 0.2a

(16.7 ± 0.8 B)

4.9 ± 1.0a

(13.9 ± 2.7A)

5.8 ± 0.1a

(20.1 ± 0.2C)

7.0 ± 0.4a

(28.1 ± 1.7 D)

Cu2+-reducing

212.5 ± 7.0c

123.2 ± 0.9b

14.1 ± 0.04a

(46.5 ± 0.1 B)

12.5 ± 0.6a

(35.2 ± 1.6 A)

10.2 ± 0.3a

(36.8 ± 0.9A)

15.7 ± 0.6a

(62.7 ± 2.5 C)

  1. The IC50 value is defined as the concentration for a 50% effect and is expressed as the mean ± SD (n = 3). The IC50 values without parentheses are in μg/mL units; The IC50 values in parentheses are in μM units. Mean IC50 values in μg/mL units with different superscripts (a, b) in the same row are significantly different (p < 0.05), while those with the same superscripts are not significantly different (p < 0.05). Mean IC50 values in μM units with different superscripts (A, B) in the same row are significantly different (p < 0.05), while those with the same superscripts are not significantly different (p < 0.05). FRAP ferric ion reducing antioxidant power; ABTS, 2,2′-azino-bis (3-ethylbenzo-thiazoline-6-sulfonic acid; DPPH, 1,1-diphenyl-2-picryl-hydrazl; PTIO, 2-phenyl-4,4,5,5-tetramethylimidazoline-1-oxyl 3-oxide; LAMF, lyophilized aqueous extract of Mori Fructus; lyophilized aqueous extract of Mori Ramulus. Trolox acted as the positive control. The dose-response curves are listed in Additional file 1