Fig. 3

JC-001 mediates cytokine secretion under co-culture conditions. The effector cell (LLC1 immunized splenocytes) to target cell (LLC1 cells) ratio was 40, and each bar represents the mean ± standard deviation (n = 3). *p < 0.05, **p < 0.01 compared with the control group. After co-culture for 48 h, JC-001 up-regulated IL-2, IL-10, TNF-α and IFN-γ and down-regulated IL-17A, and TGF-β in the condition medium. The results are expressed as mean ± SD of three separate experiments