Fig. 7

Isoliquiritigenin inhibits LPS-induced NO formation and HMGB1 release in RAW264.7 cells. a Cells cultured for 24 h in serum-free medium were stimulated with vehicle (DMSO) or LPS in the presence of DOE, isoliquiritigenin, dalbergin, or sativanone. After incubation for 24 h, aliquots of conditioned media were subjected to nitrite analysis. b Cells cultured for 24 h in serum-free medium were triggered by vehicle (DMSO) or LPS in the absence or presence of isoliquiritigenin. After incubation for 24 h, equal volumes of conditioned medium were subjected to nitrite analysis or immunoblot analysis to determine the levels of HMGB1. Ponceau S staining was used as a loading control. The results are plotted as the means ± S.E. (n = 6). ^*, p < 0.01 vs untreated group; ^#, p < 0.01 vs LPS-treated group