Fig. 4

DOE inhibits LPS-triggered iNOS expression and NO formation in RAW 264.7 cells. a Cells cultured for 24 h in serum-free medium were triggered by vehicle (DMSO) or LPS. DOE was added at the indicated time points after LPS treatment for 24 h. The levels of nitrite were determined by incubation with Griess reagents. b Cells cultured for 24 h in serum-free medium were triggered by vehicle (DMSO) or LPS with or without indicated concentrations of DOE. After incubation for 24 h, aliquots of whole-cell lysates or conditioned media were subjected to immunoblot or nitrite analysis, respectively. The results are plotted as the means ± S.E. (n = 6). ^*, p < 0.01 vs untreated group; ^#, p < 0.01 vs LPS-treated group