Fig. 3From: A Dalbergia odorifera extract improves the survival of endotoxemia model mice by inhibiting HMGB1 releaseTreatment with DOE after LPS exposure attenuates LPS-triggered release of HMGB1 in RAW 264.7 cells. Cells cultured for 24Â h in serum-free medium were triggered by vehicle (DMSO) or LPS. DOE was added at the indicated time points after LPS treatment for 24Â h. Equal volumes of conditioned medium were subjected to immunoblot analysis to determine the levels of HMGB1. As a loading control, Ponceau S staining was used. The results are representative of three or four independent experimentsBack to article page