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Fig. 3 | BMC Complementary and Alternative Medicine

Fig. 3

From: A Dalbergia odorifera extract improves the survival of endotoxemia model mice by inhibiting HMGB1 release

Fig. 3

Treatment with DOE after LPS exposure attenuates LPS-triggered release of HMGB1 in RAW 264.7 cells. Cells cultured for 24 h in serum-free medium were triggered by vehicle (DMSO) or LPS. DOE was added at the indicated time points after LPS treatment for 24 h. Equal volumes of conditioned medium were subjected to immunoblot analysis to determine the levels of HMGB1. As a loading control, Ponceau S staining was used. The results are representative of three or four independent experiments

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