Fig. 4

AAE regulates cytochrome c translocation to the cytoplasm and Bax translocation to the mitochondrial membrane. a AAE regulated cytochrome C translocation from mitochondria to cytosol. Also, (b) AAE induced BAX activation from mitochondria outer membrane to cytosol and BAX/BAK oligomerization. Cell were treated with AAE (40ìg/ml) for 24 h. Target protein and mitochondria were stained with fluorescence lag and cell locations were indicated using a DIC image