Fig. 6

Protective effects of C. crepidioides extract on the cell survival in alloxan-treated INS-1 cells by Hoechst 33,342 staining method. a Representative images by Hoechst 33,342 staining, arrowheads in the pictures indicate the nuclei of the apoptotic cells (Hoechst-positive cells). b The apoptosis rate was determined by calculating the percentage of Hoechst positive cells over the total number of cells. Values were represented as mean ± SD from three independent experiments. ## p < 0.01 as compared with the control group; **p < 0.01 as compared with the alloxan control group. Apoptosis activity results for INS-1 cell line induced with 200 μM of alloxan and pre incubated with different concentrations of extract