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Table 8 MGIT-DST of PZA R-Mtb with DFO-B alone and in combination with PZA

From: Preliminary evaluation of anti-tuberculosis potential of siderophores against drug-resistant Mycobacterium tuberculosis by mycobacteria growth indicator tube-drug sensitivity test

Mtb Isolate DFO-B (mg/mL) DFO-B (mg/mL) + PZA 100 μg/mL Ferric citrate + DFO-B (mg/mL)
0.25 0.5 1.0 0.25 0.5 1.0 1
PZA R isolate 1 209 41 0 0 0 0 400
PZA R isolate 2 129 0 0 0 0 0 400
MDR + PZA R isolate 3 400 400 0 7 0 0 400
MDR + PZA R isolate 4 400 400 179 400 400 400 400
MDR + PZA R isolate 5 400 400 0 0 0 0 400
MDR + PZA R isolate 6 400 400 400 400 400 400 400
MDR + PZA R isolate 7 400 400 0 400 400 0 400
INFERENCE R S n = 2 S n = 5 S n = 4 S n = 4 S n = 5 R
  1. GU value of GC for all seven isolates was 400. GU value in PZA-containing medium for all isolates was also 400. R resistant, S susceptible. If the GU value of the drug-containing tube is ≥100, the interpretation is Resistant by the MGIT instrument. If the GU value of the drug tube is <100, the interpretation is Susceptible (n = no. of isolates susceptible)
  2. Inhibitory effect of DFO-B and DFO-B + PZA on seven PZA R isolates: The medium used was Middlebrook 7H9 broth, pH = 5.9 incubated at 37 °C for 14 days in the MGIT instrument