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Table 6 MGIT-DST of MDR-Mtb with DFO-B alone and in combination with INH and RIF

From: Preliminary evaluation of anti-tuberculosis potential of siderophores against drug-resistant Mycobacterium tuberculosis by mycobacteria growth indicator tube-drug sensitivity test

MDR- Mtb (INH R + RIF R) Isolate DFO-B (mg/mL) DFO-B (mg/mL) + INH 0.1 μg/mL DFO-B (mg/mL) + RIF 1.0 μg/mL Ferric citrate + DFO-B (mg/mL)
0.25 0.5 1.0 0.25 0.5 1.0 0.25 0.5 1.0 1.0
1 400 400 0 400 400 0 400 400 0 400
2 400 400 0 400 400 0 400 400 0 400
3 400 0 0 400 0 0 400 0 0 400
4 400 0 0 400 0 0 400 0 0 400
5 400 400 400 400 400 400 400 400 400 400
6 400 400 0 400 0 0 400 0 0 400
7 400 400 0 400 400 0 400 400 0 400
8 400 0 0 400 0 0 400 0 0 400
9 400 0 0 400 0 0 400 0 0 400
10 400 400 0 400 400 0 400 400 0 400
INFERENCE R S n = 4 S n = 9 R S n = 5 S n = 9 R S n = 5 S n = 9 R
  1. GU value of GC for all ten isolates was 400. GU value in INH- and RIF-containing medium for all isolates was also 400. R resistant, S susceptible. If the GU value of the drug-containing tube is ≥100, the interpretation is Resistant by the MGIT instrument. If the GU value of the drug tube is <100, the interpretation is Susceptible (n = no. of isolates susceptible)
  2. Inhibitory effect of DFO-B, DFO-B + INH, DFO-B + RIF on ten different clinical isolates of MDR-Mtb (INH-, RIF- and PZA-resistant). The medium used was Middlebrook 7H9 broth, pH = 7 incubated at 37 °C for 14 days in the MGIT instrument