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Table 1 Protocol for MGIT-DST of MDR-Mtb with Exo-MS alone and in combination with INH and RIF

From: Preliminary evaluation of anti-tuberculosis potential of siderophores against drug-resistant Mycobacterium tuberculosis by mycobacteria growth indicator tube-drug sensitivity test

No Drug Initial Concentration of the drug preparation Volume added to MGIT for Test Final Concentration in MGIT tube except GC No. of Middle Brook tubes
GC MDR
1 INH 8.3 μg/mL 100 μL 0.1 μg/mL 1 1
2 RIF 83 μg/mL 100 μL 1.0 μg/mL   1
3 Exo-MS + INH 19 mg/mL + 8.3 μg/mL 50 μL + 100 μL 0.125 mg/mL and 0.1 μg/mL   1
4 Exo-MS + INH 19 mg/mL + 8.3 μg/mL 100 μL + 100 μL 0.25 mg/mL and 0.1 μg/mL   1
5 Exo-MS + INH 19 mg/mL + 8.3 μg/mL 200 μL + 100 μL 0.5 mg/mL and 0.1 μg/mL 1 1
6 Exo-MS + RIF 19 mg/mL + 83 μg/mL 50 μL + 100 μL 0.125 mg/mL and 1.0 μg/mL   1
7 Exo-MS + RIF 19 mg/mL + 83 μg/mL 100 μL + 100 μL 0.25 mg and 1.0 μg/mL   1
8 Exo-MS + RIF 19 mg/mL + 83 μg/mL 200 μL + 100 μL 0.5 mg and 1.0 μg/mL   1
9 Exo-MS 19 mg/mL 50 μL 0.125 mg/mL 1 1
10 Exo-MS 19 mg/mL 100 μL 0.25 mg/mL   1
11 Exo-MS 19 mg/mL 200 μL 0.5 mg/mL   1
12 Exo-MS+ FeCl3 19 mg/mL + 80 mg/mL 200 μL + 50 μL 0.5 mg/mL and 0.5 mg/mL   1